Engineering an incubation environment that mimics in situ conditions for in vitro coastal microbiome studies

Author:

Hunter Brad T1ORCID,Flury Jaeden D1ORCID,Cocioba Sebastian S2ORCID,Cope-Arguello Matthew L1ORCID,Helms Jared M1ORCID,García Kevin H1ORCID,Dominguez Gerardo1ORCID,Taniguchi Darcy AA1ORCID,Becket Elinne1ORCID

Affiliation:

1. College of Science, Technology, Engineering, & Mathematics, California State University San Marcos, 333 S. Twin Oaks Valley Rd, San Marcos, CA 92096, USA

2. Binomica Labs, Long Island City, NY, 4301 22nd St fl 3rd studio 342, Long Island City, NY 11101, USA

Abstract

Coastal environments are dynamic and can vary widely on short- or long-term scales depending on location and weather. Incubation equipment that reflects these changes through programmable gradient light and temperature cycles would permit more precise in vitro coastal microbiome studies. Here we present an open-source incubation environment that mimics in situ conditions for in vitro coastal microbiome studies using a modified shaking water bath that has fully customizable temperature and light gradients that can also mimic real-time field conditions. We compared coastal microbial community profiles incubated in situ and in our build mimicking field conditions over 48 h. Analyses of congruence indicated significant overlap (p > 0.2) between microbial communities incubated in situ and in vitro at each time point.

Funder

National Institute of General Medical Sciences

U.S. Department of Education

National Institute of Environmental Health Sciences

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3