SUMOylation of the chromodomain factor MRG-1 in C. elegans affects chromatin-regulatory dynamics

Author:

Baytek Gülkiz12ORCID,Blume Alexander123,Demirel Funda Gerceker12,Bulut Selman123,Popp Oliver1,Mertins Philipp1,Tursun Baris123ORCID

Affiliation:

1. Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, 13125, Germany

2. Berlin Institute for Medical Systems Biology (BIMSB), Berlin, 10115, Germany

3. University of Hamburg, Department of Biology, Institute of Cell & Systems Biology of Animals, Hamburg, 20146, Germany

Abstract

Epigenetic mechanisms control chromatin accessibility and gene expression to ensure proper cell fate specification. Histone proteins are integral chromatin components, and their modification promotes gene expression regulation. Specific proteins recognize modified histones such as the chromodomain protein MRG-1. MRG-1 is the Caenorhabditis elegans ortholog of mammalian MRG15, which is involved in DNA repair. MRG-1 binds methylated histone H3 and is important for germline maturation and safeguarding. To elucidate interacting proteins that modulate MRG-1 activity, we performed in-depth protein–protein interaction analysis using immunoprecipitations coupled with mass spectrometry. We detected strong association with the Small ubiquitin-like modifier SUMO, and found that MRG-1 is post-translationally modified by SUMO. SUMOylation affects chromatin-binding dynamics of MRG-1, suggesting an epigenetic regulation pathway, which may be conserved.

Funder

H2020 European Research Council

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

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