Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites-Reference-Cited by-同舟云学术

Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites

Published:2022-03 Issue:3 Volume:72 Page:85-89
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Bailey Dustin W¹^ORCID,Attia Ziv¹^ORCID,Reinert Stephan¹²^ORCID,S Hulke Brent³^ORCID,Kane Nolan C¹^ORCID

Affiliation:

1. Ecology & Evolutionary Biology Department, University of Colorado, Boulder, CO 80309, USA

2. Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, 91058, Erlangen, Germany

3. Sunflower and Plant Biology Research Unit, USDA-ARS Edward T. Schafer Agricultural Research Center, Fargo, ND 58102, USA

Abstract

The Asteraceae are the largest plant family but among the least studied at the genome level. Our work investigated practical methods to reduce the influence of secondary metabolites – specifically, phenolic compounds – on the extraction of DNA from Silphium spp. This genus is in the Heliantheae tribe of Asteraceae that also includes sunflower ( Helianthus annuus). Three methods were attempted in Silphium, with varying condition and age of the leaf sample. A modified cetyl trimethylammonium bromide (CTAB) method on young leaves resulted in the best DNA yield, with sufficient sample purity. No perceptible difference was observed between fresh and lyophilized samples for any extraction method or leaf age. These results provide an excellent basis for DNA extraction of difficult plant samples.

Funder

The Perennial Agriculture Project

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/btn-2021-0050

Reference19 articles.

1. Cupplant Silage as a Replacement for Corn Silage in Growing Beef Cattle Diets

2. Botanical characteristics, crop management and potential of Silphium perfoliatum L. as a renewable resource for biogas production: A review

3. Strategic use of native species on environmental gradients increases diversity and biomass relative to switchgrass monocultures

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Two new species of Pustula (Albuginales, Oomycota) from northern Iran;Mycological Progress;2023-10-04

2. A DNA Extraction Method for Nondestructive Testing and Evaluation of Cotton Seeds (Gossypium L.);Biochemical Genetics;2023-08-21

3. The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.);Plants;2023-07-11

4. Balancing read length and sequencing depth: Optimizing Nanopore long‐read sequencing for monocots with an emphasis on the Liliales;Applications in Plant Sciences;2023-05

5. A DNA extraction method for nondestructive testing and evaluation of cotton seeds (Gossypium L.);2023-04-11