A cell-based assay for the detection of neutralizing antibodies against alemtuzumab-Reference-Cited by-同舟云学术

A cell-based assay for the detection of neutralizing antibodies against alemtuzumab

Published:2020-04 Issue:4 Volume:68 Page:185-190
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Ali Liaqat¹²,Saxena Gauri¹,Jones Meleri¹,Leisegang Georgia R³,Gammon Luke¹,Gnanapavan Sharmilee⁴⁵,Giovannoni Gavin⁴⁵,Schmierer Klaus⁴⁵,Baker David⁴,Kang Angray S³^ORCID

Affiliation:

1. Barts & The London School of Medicine & Dentistry, Blizard Institute, Queen Mary University of London, Whitechapel, London, UK

2. Department of Biological Sciences, National University of Medical Sciences (NUMS), Rawalpindi 46000, Pakistan

3. Centre for Oral Immunobiology & Regenerative Medicine, Institute of Dentistry, Barts & the London School of Medicine & Dentistry, Queen Mary University of London, London, E1 2AT, UK

4. BartsMS, Blizard Institute, Barts & The London School of Medicine & Dentistry, Queen Mary University of London, London, E1 2AT, UK

5. Clinical Board: Medicine (Neuroscience), The Royal London Hospital, Barts Health NHS Trust, London, E1 1BB, UK

Abstract

Aim: The humanized anti-CD52 monoclonal antibody alemtuzumab depletes lymphocytes and is currently used to treat relapsing multiple sclerosis. During treatment, anti-alemtuzumab antibodies may develop and reduce effective lymphocyte depletion in future treatment cycles. Results: Alemtuzumab–Alexa Fluor 488 conjugate binding to the CHO-CD52 cell surface was inhibited by anti-alemtuzumab antibodies. Conclusion: In this proof-of-concept study, a CHO-CD52 cell line has been developed and used to detect the presence of anti-alemtuzumab neutralizing antibodies. This platform provides the basis of an assay for routine screening of serum for neutralizing antibodies from patients treated with alemtuzumab.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/btn-2019-0122

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Detecting and predicting neutralization of alemtuzumab responses in MS;Neurology - Neuroimmunology Neuroinflammation;2020-06-04

2. The Meaning of Immune Reconstitution after Alemtuzumab Therapy in Multiple Sclerosis;Cells;2020-06-03