Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation-Reference-Cited by-同舟云学术

Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation

Published:2018-09 Issue:3 Volume:65 Page:143-148
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Fouda Ahmed E¹,Embogama D Maheeka¹,Ramanayake-Mudiyanselage Vindya¹,H Pflum Mary Kay¹

Affiliation:

1. Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, MI 48202, USA

Abstract

Kinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic polymer deacetylated chitosan to permeabilize ATP analogs for live cell applications, including kinase-catalyzed biotinylation.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/btn-2018-0076

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