Mammalian cell culture density determination using a laser through-beam sensor

Author:

Rue Sarah M1,Anderson Paul W12,Miller Jessica M1,Fanale Salvatore G1,Chang Jim Y1,Glaser Scott M1,Lesley Scott A13

Affiliation:

1. Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA

2. Lilly Biotechnology Center, San Diego, CA 92121, USA

3. Merck Research Laboratories, Palo Alto, CA 94304, USA

Abstract

High-throughput protein expression platforms are increasingly used to produce proteins for many applications: to support studies in structure/function, regulation and proteomics, as well as for direct use as potential biotherapeutic agents for medical applications. Here we describe a device that we refer to as the flask density reader (FDR) consisting of a through-beam laser and sensor, and a customized culture flask-receiving nest. The FDR has been integrated onto GNF System™'s automated protein expression platform to enable rapid, noninvasive, fully automated spectrophotometric determination of cell densities in suspension mammalian cell cultures. The FDR reduces the risk of culture contamination from frequent flask sampling and greatly reduces the time and effort needed to count cells using off-line methods.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

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