Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer-Reference-Cited by-同舟云学术

Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer

Published:2009-01 Issue:1 Volume:46 Page:58-60
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Intemann Christopher D.¹²,Thye Thorsten¹²,Sievertsen Jürgen¹,Owusu-Dabo Ellis³⁴,Horstmann Rolf D.¹,Meyer Christian G.¹

Affiliation:

1. Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany

2. University Hospital Schleswig-Holstein, Campus Lübeck, Institute of Medical Biometry and Statistics, Lübeck, Germany

3. Department of Community Health, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

4. Kumasi Centre for Collaborative Research in Tropical Medicine, Kumasi, Ghana

Abstract

Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West Africa.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/000113045

Cited by 9 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Among autophagy genes, ATG16L1 but not IRGM is associated with Crohn's disease in Iranians;Gene;2018-10

2. Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt (HRM) analysis;F1000Research;2014-02-26

3. Rapid and inexpensive body fluid identification by RNA profiling-based multiplex High Resolution Melt (HRM) analysis;F1000Research;2013-12-20

4. In VitroFRET Sensing, Diagnostics, and Personalized Medicine;FRET – Förster Resonance Energy Transfer;2013-10-04

5. An STR Melt Curve Genotyping Assay for Forensic Analysis Employing an Intercalating Dye Probe FRET*;Journal of Forensic Sciences;2010-09-14