Affiliation:
1. City of Hope National Medical Center, Duarte, CA, USA
Abstract
To measure mutation load or to detect minimal residual disease, a robust method for identifying one mutant allele in the range of 106–109 wild-type alleles would be advantageous. Herein, we present evidence that pyrophosphorolysis-activated polymerization (PAP) has the potential to provide a highly specific and robust method of allelespecific amplification if DNA polymerases with higher pyrophosphorolysis activity can be found or engineered. In PAP, pyrophosphorolysis and polymerization by DNA polymerase are coupled serially by utilizing a pyrophosphorolysis-activatable oligonucleotide (P*). P*, which is an allele-specific oligonucleotide with a dideoxynucleotide at the 3′ terminus, can be activated by pyrophosphorolysis to remove the 3′ terminal dideoxynucleotide in the presence of pyrophosphate (PPi) and the complementary strand of the allelic template; then the activated P* can be extended by DNA polymerization. Specificity results from both pyrophosphorolysis and polymerization because significant nonspecific amplification requires the combination of mismatch pyrophosphorolysis and misincorporation by the DNA polymerase, which is an extremely rare event. Proof of principle has been achieved with a polymorphic site within the human D1 dopamine receptor gene. The effects of the dideoxyoligonucleotide sequences, DNA polymerases, PPi concentrations, allele-specific templates, pH and dNTP concentrations were examined.
Subject
General Biochemistry, Genetics and Molecular Biology,Biotechnology
Cited by
57 articles.
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