Low titer lentiviral transgenesis in rodents with simian immundeficiency virus vector-Reference-Cited by-同舟云学术

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Low titer lentiviral transgenesis in rodents with simian immundeficiency virus vector

Published:2013-09 Issue:3 Volume:55 Page:137-140
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Bender Balázs¹²,Hoffmann Orsolya Ivett¹,Negre Didier³,Kvell Krisztián⁴,Bösze Zsuzsanna¹,Hiripi László¹

Affiliation:

1. Institute of Animal Biotechnology, Agricultural Biotechnology Center, Gödöllö, Hungary

2. ImmunoGenes Kft, Budakeszi, Hungary

3. Inserm, U758, Human Virology Department, Université de Lyon, Ecole Normale Supérieure de Lyon, Lyon, France

4. Department of Immunology and Biotechnology, University of Pécs, Pécs, Hungary

Abstract

Efficient production of transgenic animals using low-titer lentiviral constructs remains challenging. Here we demonstrate that microinjection of simian immundeficiency virus-derived lentiviral constructs can produce transgenic mice and rats with high efficiency even when using low-titer virus preparations.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/000114078

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