Micro-Perfusion Flow Cell for Imaging Cultured Cells

Author:

Agnihotri Naveen1,Kisaalita William S.1,Keith Charles H.1

Affiliation:

1. University of Georgia, Athens, GA, USA

Abstract

We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short working distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin®, the flow cell is biologically inert. We have used the flow cell for fluorescence imaging of PC12 cells loaded with tetramethylrhodamine dextran (TMRD) and other dyes.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

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