Affiliation:
1. JSC Avicenna medical centre of the group of companies "Mother and Child"; State Budgetary Educational Institute of Higher Professional Education «Novosibirsk State Medical University of Ministry of Health» of Russia)
2. Autonomous Public Health Care Institution of the Novosibirsk Region "City clinical polyclinic No. 1»
3. Institute of Molecular and Cellular Biology of the Siberian Branch of the RAS; JSC "Vector-Best"
4. Private medical institution "Russian Railways-Medicine" Clinical hospital"
5. JSC Avicenna medical centre of the group of companies "Mother and Child"
Abstract
miRNA and mRNA are highly specific molecular markers, with their own unique expression profile for each type of tissue. They can become a valuable tool in clinical practice in addition to routine esophagogastroduodenoscopy.Purpose. To study the prospects of using a classifier based on miRNA and mRNA profiling in cytological samples for detecting dysplasia and stomach cancer, as well as to compare the obtained data with the results of profiling of 7 miRNAs used for the analysis of both histological and cytological material.Materials and methods. The study included 221 cytological preparations: 108 samples of adenocarcinoma, 27 samples of dysplasia, 86 samples of normal mucosa. The expression level of miRNA-145-5p, -150-5p, -21,-20a, -31-5p, -34a-5p, -375, -125b, -196b, -106b and mRNA of the following genes: TERT, CDKN2A, CKS2, FN1, was determined using real-time reverse transcription polymerase chain reaction. Quantitative comparison of two independent samples for was performed using the Mann-Whitney test. The samples were stratified into different groups using the C5.0 algorithm.Results: when examining cytological samples in cases of miRNA-125b, 145, -196b, -21, -375 and TERT, СKS2, FN1 mRNA, there was achieved a high level of significance of differences in the cancer/norm group; miRNA-375 and FN1 mRNA in the cancer/dysplasia group and miRNA-145, -196b, -20a, and СKS2, TERT mRNA in the norm/dysplasia groups. When comparing the results for the 7 miRNAs that were used to analyze both histological and cytological material, several differences was noted.Conclusion. The practical use of miRNA and mRNA expression (in cytological material data mining technology does not allow differentiating dysplasia and cancer but can help in identifying "high-risk patients" who should repeat esophagogastroduodenoscopy with multifocal forceps biopsy, with mandatory molecular genetic research.
Publisher
Center of Endourology Endocenter
Reference16 articles.
1. Gastric Cancer. Clinical Recommendations. Ministry of Health of the Russian Federation; 2018. 34 p. (in Russian)
2. Yada T., Yokoi C., Uemura N. The current state of diagnosis and treatment for early gastric cancer. Diagnostic and Therapeutic Endoscopy. 2013;2013:241320. https://doi.org/10.1155/2013/241320
3. Avtandilov G.G., Kupryushina N.V. Histoploidometry diagnostics of gastric neoplasms in biopsy samples. Moscow: Izdanie RMAPO; 2008.126 (in Russian).
4. State of cancer care to the population of Russia in 2019 ed. Caprin A.D., Starinsky V.V., Shahzadova А.О. Oncology Research Center. 2020; 239. (In Russian)
5. Kwack W.G., Ho W.J., Ho W.J., Kim J.H., Lee J.H., Kim E.J., Kang H.W., et al. Understanding the Diagnostic Yield of Current Endoscopic Biopsy for Gastric Neoplasm: A Prospective Single-Center Analysis Based on Tumor Characteristics Stratified by Biopsy Number and Site. Medicine (Baltimore). 2016;95(30):4196. https://doi.org/10.1097/MD.0000000000004196
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献