Administration of Recombinant Platelet Derived Growth Factor-Bb (PDGF-Bb) Intrafracture on Shaft Femur Rat Wistar Performed Osteotomy and Intramedullary Wire Improves Healing Process of Fractures, Collagen Type I And III Levels

Abstract

Introduction: Of all the fractures that can occur in human body, one of the most lethal is femoral fracture. Treatment of femoral fracture can be divided into two, which is operative and non-operative. External fixation, intramedullary nailing, locked intramedullary nail, and plate and screw are the options for operative treatment. Platelet Derived Growth Factor (PDGF-BB) have essential role of wound healing, due to positive effect of upregulating proliferation of fibroblast, synthesize of extracellular matrix, and revascularization. In this study we aimed to see the effect of PDGF-BB fracture healing process, type I and III collagen levels in the Wistar rats femoral shaft treated with osteotomy and intramedullary nailing. Methods: This study is an experimental randomized post-control only to animal with femoral shaft osteotomy and intramedullary wire installation in Wistar Rats that added PDGF-BB intrafracture, to the collected data then analyzed using Pearson test in SPSS 25. Results: Based on the independent t test result, we found that callus formation, type I and III collagen expression on the femur shaft of wistar rats that has been administered PDGF-BB intrafracture had a statistically significant result compared to those who had not been administered PDGF-BB intrafracture with P<0.05. Conclusion: Mean Allen score, type I collagen and type III collagen levels of shaft femur in Wistar rats group that been osteotomized and attached by intramedullary wire added with PDGF-BB is higher compared to non PDGF-BB.