EVALUATION OF IN VIVO ANTIOXIDANT ACTIVITY OF HYDRO ALCOHOLIC EXTRACT OF LINUM USITATISSIMUM L. (TUKHM-E-KATAN) AGAINST HIGH FAT DIET INDUCED RATS

Abstract

The aim of the present study was to evaluate the in vivo antioxidant activity of 50% ethanolic extract of Linum usitatissimum against high fat diet induced rats. Animals were treated with plant extract for 30 days, and high fat diet was given to all groups except plain control through, out the study, and alpha tocopherol acetate (Vit, E) was used as standard. pre-treatment with 23 mg/100 gm of body weight of 50% ethanolic extract of Linum usitatissimum significantly improved the superoxide dismutase, catalase, glutathione, and lipid peroxidation levels as compared to control group. The present studies revealed that the in vivo antioxidant activity of Linum usitatissimum was significant, and can be used to protect tissue from oxidative stress. The result showed that the superoxide dismutase, catalase, lipid peroxidase, and glutathione reductase activities significantly declined in group treated with high fat diet than that of normal group. Based on this investigation, it was concluded that the 50% ethanolic extract of Linum usitatissimum has good in vivo antioxidant activity and can be used in protecting tissue from oxidative stress.