Author:
Sharma Rajesh, ,Sharma Mukesh C.,Vijaywargiya Gaurav
Abstract
Chromatographic separation of paracetamol, aceclofenac and tramadol hydrochloride was performed on a Chromatopak C-18 column (25 cm x 4.6mm i.d. x 5µm) as stationary phase with a mobile phase composed of phosphate buffer pH 7.0: acetonitrile (65:35 V/V), pH 7.0 (adjusted with triethylamine) at flow rate of 1mL/min. Detection was carried out at 265 nm. The retention times of paracetamol, aceclofenac and Tramadol hydrochloride were found to be 2.7, 4.5 and 6.0 min, respectively. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. The method was found to be accurate, precise, specific, robust, and linear for the determination of paracetamol, aceclofenac and tramadol hydrochloride in pharmaceutical dosage form.
Publisher
Indian Drug Manufacturers' Association (IDMA)
Subject
Drug Discovery,Pharmaceutical Science,Pharmacology
Reference21 articles.
1. 1. Ravisankar S., Vasudevan M. and Najan M J.: Reverse phase HPLC method for the estimation of Paracetamol, Chlorzoxazonze, and Diclofenac sodium in formulations. Ind.Drug 1997,34(11) 663-65.
2. 2. Shenoy K.R.P., Krishnamurthy K.S. and Sumatheendra K.S.: Determination of Codeine phosphate, oxylamine succinate, Paracetamol and Caffeine in combined dosage formulation by reverse phase liquid chromatography. Ind. Drug. 2000,37(10),486-88.
3. 3. Vaidya V.V., Singh G.R., Choukekar M.P.and Kekare M.B.: Simultaneous RP HPLC determination of aceclofenac, paracetamol and tizanidine in pharmaceutical preparations. E. J. Chem. 2010,7,260-264.
4. 4. Pawar U.D., Naik A.V., Sulebhavikar A.V., Datar T.A. and Mangaonkar K.V.: Simultaneous determination of aceclofenac, paracetamol and chlorzoxazone by HPLC in tablet dose form. E. J. Chem. 2009,6,289-294.
5. 5. Vasudevan M., Ravisankar S., Ravibabu T. and Nanjan M.J. Simultaneous estimation of Paracetamol, Methocarbamol and Ibuprofen by reversed phase HPLC method. Ind.Drug. 2000,37(8),386-89.