Author:
Jadeja Priyanka,Patel Jaimin S.,Shah Dimal A.,Patel Vandana B.,
Abstract
A specific, accurate, precise, and reproducible liquid chromatographic method has been developed and validated for the estimation of acamprosate calcium and baclofen in combination. The separation was achieved using stationary phase Phenomenex C18 column (150 mm× 4.6 mm.) in isocratic mode, with mobile phase containing 0.05 M potassium dihydrogen orthophosphate buffer (pH 7) : acetonitrile (10:90 V/V), at a flow rate of 1.0 mL/min and effluents were monitored at 210 nm. The retention time of acamprosate calcium and baclofen were found to be 1.9 min and 5.3 min, respectively. The linearity for acamprosate calcium and baclofen were in the range of 2-64 µg/mL and 1.2- 38.4 µg/mL, respectively. The method was validated as per ICH guideline. The recoveries of Acamprosate calcium and baclofen were found in the range of 98.90 - 100.13 % and 98.60 -100.02 %, respectively. The method was successfully applied for the determination of both the drugs in combination.
Publisher
Indian Drug Manufacturers' Association (IDMA)
Subject
Drug Discovery,Pharmaceutical Science,Pharmacology
Reference29 articles.
1. 1. Indian Pharmacopoeia, Govt. of India Ministry of health and Family Welfare, 2nd Edn, The Indian Pharmacopoeia Commission, Ghaziabad, 2014, pp 976-972,1131-1134.
2. 2. Tripathi KD, Essentials of medical pharmacology, 6th Edn, Jaypee Brothers Medical Publishers Pvt. Ltd, New Delhi 2005, pp 414-417.
3. 3. Chumalav I., Nabirotchkin S., Cholet N. and Milet A.: Combining two repurposed drugs as a promising approach for Alzheimer’s disease therapy, Sci. Rep., 2015, 5, 7608.
4. 4. Rodolphe H., Damien T. and llya C.: Combination of acamprosate and baclofen as a promising therapeutic approach for Parkinson’s disease, Sci. Rep., 2015, 5, 16084.
5. 5. British Pharmacopoeia, British Pharmacopoeia Commission , volume I, London, 2016, pp 187, 1594.