DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR QUANTITATION OF CLOFARABINE-Reference-Cited by-同舟云学术

DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR QUANTITATION OF CLOFARABINE

Published:2022-03-07 Issue:01 Volume:59 Page:60-63
ISSN:0019-462X
Container-title:Indian Drugs
language:
Short-container-title:IND. DRU.

Author:

Shirman Asif, ,Khan Gulam J.,Ansari Yaasir A.,Qazi Shoeb,Inamdar Z.E.,Siddiqi Hifjurrahman,Syed Umar F.,Syed Tasqeeruddin,Syeda Shaheen

Abstract

This experiment aims to develop and validate the RP-HPLC method for clofarabine in a bulk and pharmaceutical dosage form. Chromatographic separation was achieved on Inertsil ODS 3V (150× 4.6 mn) diameter column. The mobile phase comprised of phosphate buffer (pH 4.0): methanol (40:60) at a flow rate of 1 mL min-1 and all eluents were detected at 270 nm. The runtime was 10 minutes. Calibration curves at five levels for clofarabine were linear in the range of 10-30 µg mL-1. Accuracy for clofarabine was studied in the range of 50-150 % quality control standard levels. A validated method was found to be accurate, reproducible, linear, precise, and robust.

Publisher

Indian Drug Manufacturers' Association (IDMA)

Subject

Drug Discovery,Pharmaceutical Science,Pharmacology

Link

https://www.indiandrugsonline.org//download/January_2022_59_1_60-63.pdf

Reference14 articles.

1. 1. Kasture AV., Mahadik KR., Wododkar SG, and More HN, A Text Book of Pharmaceutical Analysis, 17th Edition, Nirali Prakashan, Pune 2002, pp. 48-57.

2. 2. Sethi PD, HPLC-Quantitative Analysis of Pharmaceutical Formulation, CBS Publishers & Distributors, New Delhi 1996, pp. 3-46.

3. 3. Chatwal GR, Instrumental Method of Chemical Analysis, Part-1, Himalaya Publishing House, 5th Edition, 2002, pp. 2.624-2.631.

4. 4. Robinson JW, Skelly Frame EM, and Frame GM, Undergraduate Instrumental Analysis, 6th Edition, Marcel Dekker 2005, pg. 806.

5. 5. Ahuja S and Scypinski S, Handbook of Modern Pharmaceutical Analysis, Academic Press 3, 2001, pp. 356-367.