PHYTOCHEMICAL ANALYSIS AND IN VITRO GENOTOXICITY, CYTOTOXICITY, ANTIOXIDANT, ANTIMICROBIAL, ANTIOBESITY ACTIVITY OF MORINDA TINCTORIA ROXB.

Abstract

The medicinal plant Morinda tinctoria Roxb. is used in the treatment of various diseases traditionally. Report on bioactive evaluation of M. tinctoria Roxb. has led us to the phytochemical examination of M. tinctoria leaves. Our objective was to isolate compounds form M. tinctoria Roxb. and characterize each compound. The isolated compounds were tested for antioxidant, antimicrobial, antiobesity, cytotoxicity and genotoxicity properties. For this work, we have chosen solvents with increasing polarities. Hexane, chloroform, ethyl acetate and methanol were used in this study. The solvent extracts were analyzed using column chromatography and the isolated compounds were characterized using FTIR, 1 H NMR, 13C NMR and mass spectra. The constituents have been characterized and screened for in vitro antioxidant, antimicrobial, antiobesity, cytotoxicity and genotoxicity properties. Nine bioactive compounds were isolated and identified: palmitic acid, fucoxanthine, broussin, isoliquiritigenin, cynarin, gallic acid, β-sitosterol, quercetin and oleuropein. The results thus indicate conclusively that M. tinctoria leaves possess various potent bioactive compounds and is therefore recommended as a plant of phytopharmaceutical importance.