RP-HPLC METHOD FOR SIMULTANEOUS QUANTIFICATION OF BERBERINE CHLORIDE AND GALANGIN IN TINOSPORA CORDIFOLIA M. AND ALPINIA GALANGA L. AND THEIR FORMULATIONS

Abstract

Berberine chloride is a quaternary ammonium compound obtained from Tinospora cordifolia M. and galangin is a member of the flavonol class of flavonoids from Alpinia galanga L. They are reported to have various pharmacological activities. The separation was carried out by using Hypersil BDS 100 RP C18 (5 µm, 250×4.6 mm i.d.) column using an isocratic mobile phase comprising acetonitrile: 10 mM phosphate buffer (45:55 V/V) pH 4.5 adjusted with orthophosphoric acid. The flow rate was maintained at 1.50 mL min-1. The detection was carried out at 365 nm. The method was validated according to ICH guidelines. The retention times of berberine chloride and galangin were found to be 3.41 and 14.13 min, respectively. The developed RP-HPLC method was found to be simple, accurate, precise, sensitive and specific for the simultaneous quantification of berberine chloride and galangin in T. cordifolia M. and A. galanga L., respectively, and their herbal formulations.