EVALUATION OF ANTIOXIDANT, HYPOGLYCAEMIC, ANTIDIABETIC AND ALPHA AMYLASE INHIBITING ACTIVITIES OF SARGASSUM WIGHTII COLLECTED FROM THE EAST COAST OF INDIA

Abstract

This study evaluated the antioxidant, hypoglycemic, antidiabetic and alpha amylase inhibiting activities of Sargassum wightii. Antioxidant activity of ethyl acetate and methanol extracts of this seaweed was evaluated and extract having promising antioxidant activity was further studied for hypoglycaemic and antidiabetic activity in normal and alloxan induced diabetic mice, respectively, at 100 and 200mg/ kg after toxicity evaluation. Ethyl acetate extract of S. wightii (EAS) has shown promising antioxidant, hypoglycemic and antidiabetic activity. Total phenol content for EAS was 250.09 ± 4.41mg/g. Fasting plasma glucose level in normal mice was significantly (p<0.05) decreased after 6 days of EAS (200mg/ kg) treatment. EAS at 100 and 200mg/kg has significantly (p<0.05) attenuated AUC of OGTT of diabetic mice. EAS was also found to have potent in vitro alpha-amylase inhibiting property. The current findings suggest that the anti-diabetic activity of S. wightii might be due to its underlying antioxidant, hypoglycemic and alpha-amylase inhibiting property.