Author:
Mallikarjuna Rao N.,Gowri Sankar D.,
Abstract
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of ascorbic acid in health drinks. Isocratic elution at a flow rate of 0.9mL/min was employed on a symmetry C18 column (250x4.6mm, 0.5μ in particle size) at ambient temperature. The mobile phase consisted of water with acetic acid: methanol 95:5% (V/V). The UV detection wavelength was 245 nm and 20μL sample was injected. The retention time for ascorbic acid was 4.61± 0.22 min. The percentage RSD for precision and accuracy of the method was found to be less than the method which was validated as per the ICH guidelines. The method was successfully applied for routine analysis of ascorbic acid in health drinks.
Publisher
Indian Drug Manufacturers' Association (IDMA)
Subject
Drug Discovery,Pharmaceutical Science,Pharmacology
Reference11 articles.
1. 1. J.Sun,Y.F.Chu,X.Wu and R.H.Liu, Journal of the Agriculture Food chemistry,2002, 50(1) 7449.
2. 2. Robert ridgely sealock, Ruth L, The role of ascorbic acid in the oxidation of L-tyrosine by guinea pig liver extracts.: Journal of biological chemistry, 1952,196(1) 761-767.
3. 3. Formation of volatile compounds in sugar-phenylalanine and ascorbic acid-phenylalanine model systems during heat treatment, available at http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.:1981.tb15349.x/abstract accessed on Aug, 2006.
4. 4. Story of Vitamin C's chemical discovery. Accessed Jan 21, 2010.
5. 5. Davies, Michael B.; Austin, John; Partridge, David A., Vitamin C: It’s Chemistry and