CHEMOMETRIC ASSISTED UV SPECTROPHOTOMETRIC METHOD FOR QUANTIFICATION OF EMTRICITABINE AND TENOFOVIR DISOPROXIL FUMARATE-Reference-Cited by-同舟云学术

CHEMOMETRIC ASSISTED UV SPECTROPHOTOMETRIC METHOD FOR QUANTIFICATION OF EMTRICITABINE AND TENOFOVIR DISOPROXIL FUMARATE

Published:2023-09-28 Issue:09 Volume:60 Page:93-99
ISSN:0019-462X
Container-title:INDIAN DRUGS
language:
Short-container-title:IND. DRU.

Author:

Gandhi Santosh V., ,Shinde Smruti C.

Abstract

The aim of this study was to verify the ability of the UV spectrophotometric method for the simultaneous determination of emtricitabine and tenofovir disoproxil fumarate in tablet dosage form using the principal Component Regression (pCR) and partial Least Squares (pLS) multivariate calibration methods. Double beam UV-Vis spectrophotometer (Jasco V-730) with 1 cm quartz cell with 1 nm data interval and scanning speed of 400 nm min -1 was used in the study. the optimized wavelength range selected was 225-275 nm. the data obtained was processed using Unscrambler X (10.5) (64bit) software. The developed models showed good results over the concentration range of 6-36 μg mL -1 for tenofovir disoproxil fumarate and 4-24 μg mL-1 for emtricitabine with co-relation coefficient greater than 0.995 and % RSD less than 2%. the accuracy studies show % recovery within limits. The method was validated as per ICH Q2(R1) guideline.

Publisher

Indian Drug Manufacturers' Association (IDMA)

Subject

Drug Discovery,Pharmaceutical Science,Pharmacology

Link

https://www.indiandrugsonline.org//download/September_2023_60_9_93-99.pdf

Reference19 articles.

1. 1) https://en.m.wikipedia.org/wiki/tenofovirdisoproxilfumarate (Accessed on 20/08/21)

2. 2) https://go.drugbank.com/drugs/DB00300 (Accessed on 20/08/21)

3. 3) https://en.m.wikipedia.org/wiki/emtricitabine (Accessed on 20/08/21)

4. 4) https://go.drugbank.com/drugs/DB00879 (Accessed on 20/08/21)

5. 5) Anandakumar K., Kannan K. and Vetrichelvan T, Development and Validation of Emtricitabine and Tenofovir Disoproxil Fumarate in pure and fixed dose combination by UV spectrophotometry. Dig. J. Nanomater. Biostructures, 2011, 6(3) 1085-1090.