DEVELOPMENT, VALIDATION AND COMPARISON OF RP-HPLC AND UV METHODS USING STATISTICAL ANALYSIS ONE WAY ANOVA TEST FOR REPAGLINIDE IN FORMULATION

Abstract

In the present study, analytical UV and Rp-HpLC methods for repaglinide were developed for tablet formulation. UV method shows linearity in the range of 10-30 μg mL-1 with a relative coefficient of 0.9999. Linearity is shown in HpLC method in the range of 10- 30 μg mL-1 with a relative coefficient of 0.9985. each method was validated for different validation parameters like specificity, repeatability, accuracy, precision, linearity, robustness, limit of detection and limit of quantification. the results were obtained as per ICH guidelines. The developed UV and HpLC methods were compared with some available methods by statistical analysis one way ANOVA (Analysis of Variance) test, and it was found to be statistically significant