COMPARATIVE ANTIOXIDANT ACTIVITY OF HYDRO-ALCOHOLIC POLYHERBAL FORMULATIONS (HAF-I & HAF-II) IN IMMOBILISED STRESS INDUCED RAT MODEL

Abstract

The present study was undertaken to develop a method and to assess the anti-stress activity of poly herbal formulation HAF-I & HAF-II in immobilised stress induced rat model. Acute toxicity study was performed according to OECD guidelines No. 423. Sprague Dawley female rats were observed periodically for the symptoms of toxicity and death within 24 h and at regular intervals for 14 d. In anti-stress study, total 24 female rats were selected and divided into four groups of six animals each. The stress was confirmed by physiological parameters as well as malonaldialdehyde and FOX1 level measured in the blood samples of 18 animals. On the basis of these parameters, treatments were started in group III to group VI animals taken individually dose of 200 mg/kg body weight daily dose per oral administration. Our finding showed that there were significant (P<0.0001) increased MDA, FOX1 and AChE enzyme activities along with decreased (P<0.0001, P<0.001) catalase, glutathione and total thiol antioxidant enzymes levels in stress induced group as compared to control group. After treatment with respective poly-herbal drugs HAF-I & HAF-II in group III to group IV animals, these above parameters were significantly improved in all treated animals after 20 days. Based on above study, it is concluded that these poly herbal formulations are effective for management of stress but comparativly, HAF-I is more effective for the improvement of stress (MDA, FOX1, AChE) levels and anti-oxidant enzyme during stress.