Investigation of Characteristics and Behavior of Loaded Carboplatin on the, Liposomes Nanoparticles, on the Lung and Ovarian Cancer: An In-Vitro Evaluation

Abstract

Carboplatin is a chemotherapy drug which is used against some forms of cancer such as ovarian and lung carcinoma. This research was investigated to optimize and assess the Cytotoxicity of carboplatin Nanoliposomes on ovarian and lung cancer cell lines. Nanoparticles were prepared using reverse phase evaporation technique and characterized for light microscopy, spectrophotometry and drug-release properties. In the next step, A2780S and A2780CP (ovarian) and TC1 (lung) cell lines were used to determine the rate of Nano liposomal carboplatin cytotoxicity. In this research, size and zeta potentials are 244.3±16.7nm and 22.9± 1.7 mV, respectively. High encapsulation capacity (78.6±3.7%) confirmed the efficiency of the reverse phase evaporation technique. The cytotoxic effect of this Nano liposome on all cell lines was significantly increased when compared with the free drug (P<0.05). Our findings suggest that carboplatin liposomal Nanocarriers could serve as alternative chemotherapy modality for ovarian and lung cancer therapy.