Penicillium Antibiotic Effect-Reference-Cited by-同舟云学术

Penicillium Antibiotic Effect

Published:2018-09-01 Issue:7 Volume:80 Page:530-535
ISSN:0002-7685
Container-title:The American Biology Teacher
language:en
Short-container-title:

Author:

Lewis Jesse A.¹,Anderson Nadja²

Affiliation:

1. JESSE A. LEWIS is a Graduate Research Assistant in the BIOTECH Project, University of Arizona, 1041 E. Lowell St, Tucson, AZ 85721, 520-626-4664; e-mail: Jlewis2@email.arizona.edu.

2. DR. NADJA ANDERSON is Director of the BIOTECH Project, University of Arizona; e-mail: Nadja@bio5.org.

Abstract

In this lesson students will use the Penicillium chrysogenum fungus, which naturally produces the antibiotic penicillin, to investigate the effect of naturally produced antibiotics on bacteria in laboratory cultures. Students co-culture P. chrysogenum with three species of bacteria to observe differences between penicillin-resistant and penicillin-sensitive bacteria. They will normalize fungal spore suspension and bacterial culture concentrations before inoculating co-cultures. After bacteria have been exposed to the antibiotic, students will quantify culture density to determine antibiotic effect in liquid culture and on solid media. Students will learn about natural product antibiotics as well as experimental design and application.

Publisher

University of California Press

Subject

General Agricultural and Biological Sciences,Agricultural and Biological Sciences (miscellaneous),Education

Link

http://online.ucpress.edu/abt/article-pdf/80/7/530/60057/abt_2018_80_7_530.pdf

Reference12 articles.

1. American Chemical Society & Royal Society of Chemistry (ACS & RCC). (1999). The Discovery and Development of Penicillin, 1928–1945. Retrieved from https://www.acs.org/content/acs/en/education/whatischemistry/landmarks/flemingpenicillin.html

2. Andrews, M. (1931, March). Ugly green mold is food and medicine. Popular Science.

3. Instrumented quantitation of live bacteria in the presence of dead cells;Applied Microbiology,1966

4. Achievements in Public Health, 1900–1999: Control of Infectious Diseases;Centers for Disease Control and Prevention (CDC);Morbidity & Mortality Weekly Report,1999

5. Fraser, S. L., & Sinave, C. P. (2017, Sep5). Enterobacter infections Treatment & management. Retrieved from http://emedicine.medscape.com/article/216845-treatment