Affiliation:
1. PAUL L. GUY is an Associate Professor in the Department of Botany, University of Otago, New Zealand; e-mail: paul.guy@otago.ac.nz.
Abstract
In this exercise students use a simplified version of an immunoassay that relies on the nonspecific binding of proteins to nitrocellulose followed by the specific binding of antibodies to antigen. This antigen–antibody interaction is detected when a chromogenic substrate, catalyzed by the enzyme conjugated to the antibody, produces a color change. The immunoblot is less expensive and faster to perform than plastic-plate-based assays. I have used this assay for over 10 years in undergraduate courses: in ecology to estimate disease incidence; in botany to explore immunological techniques; and in biotechnology as an exercise in product development.
Publisher
University of California Press
Subject
General Agricultural and Biological Sciences,Agricultural and Biological Sciences (miscellaneous),Education
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