Assessing the Effectiveness of a Novel Microscopy Technique in Middle & High School Science Classrooms

Author:

Raven Sara1,Cevik Emel2,Model Michael3

Affiliation:

1. SARA RAVEN is an Assistant Professor of Science Education in the Department of Teaching, Learning, and Culture at Texas A&M University, College Station, TX 77843; e-mail: sraven@tamu.edu.

2. EMEL CEVIK is a doctoral student in the Department of Teaching, Learning, and Culture at Texas A&M University; e-mail: emel1980@tamu.edu.

3. MICHAEL MODEL is a Research Associate Professor in the Department of Biological Sciences at Kent State University, Kent, OH 44240; e-mail: mmodel@kent.edu.

Abstract

Although research and new technologies have introduced different ways of observing microorganisms, including scanning and electron microscopy, these methods are expensive and require equipment that is typically not found in a middle school classroom. The transmission-through-dye technique (TTD; Gregg et al., 2010), a new optical microscopy method that can be used with current basic light microscopes, relies on the fairly simple mechanism of filtered light passing through a dyed medium to produce an image that reflects cell thickness. With this technique, living microorganisms look bright red against a dark background, and movement can be seen easily among dead microorganisms and debris that show up black. Since the technique is low-cost and easy to implement, it addresses the needs of practitioners and is appropriate for a wide array of school contexts. We describe a three-week, hands-on, inquiry-based unit on TTD microscopy for middle and high school students.

Publisher

University of California Press

Subject

General Agricultural and Biological Sciences,Agricultural and Biological Sciences (miscellaneous),Education

Reference12 articles.

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2. The bioscope initiative: integrating technology into the biology classroom;American Biology Teacher,2002

3. Exploring nanoscience and scanning electron microscopy in K–12 classrooms;Microscopy Today,2015

4. Feinberg, J. (2014). Wordle. Retrieved from http://www.wordle.net.

5. Measurement of the thickness and volume of adherent cells using transmission-through-dye microscopy;Pflügers Archiv–European Journal of Physiology,2010

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