Extracting DNA to Visualize the Unity & Diversity of Life

Author:

Kinoshita Yoshihito1,Yamanoi Takahiro2,Takemura Masaharu3

Affiliation:

1. YOSHIHITO KINOSHITA (yk_an_enmachbi@yahoo.co.jp) is a graduate student in the Graduate School of Mathematics and Science Education, Tokyo University of Science (RIKADAI), Kagurazaka, Shinjuku, Tokyo, Japan.

2. TAKAHIRO YAMANOI (yamanoit@fc.hakuoh.ac.jp) is a Lecturer in the Faculty of Education, Hakuoh University, Oyama, Tochigi, Japan.

3. MASAHARU TAKEMURA (takemura@rs.kagu.tus.ac.jp) is an Associate Professor in the Graduate School of Mathematics and Science Education, Tokyo University of Science (RIKADAI), Kagurazaka, Shinjuku, Tokyo, Japan.

Abstract

We have developed a new DNA extraction experiment for high schools. It uses the concept “Life possesses not only unity, but also diversity” to teach Japanese and American students that various organisms have DNA in their bodies as a common chemical background. In this experiment, students extract DNA from representative organisms of the five kingdoms Monera, Protista, Fungi, Plantae, and Animalia (although the system of classification into five kingdoms has been replaced by three domains in current taxonomy, Japanese students are familiar with this classification system from their earlier education). After practice experiments with high school students and biology teachers were performed, the educational effects were evaluated by questionnaires. The results suggested that the DNA extraction experiments were effective to a certain extent and that, although several points should be improved, this experiment is adequate for practice in high schools.

Publisher

University of California Press

Subject

General Agricultural and Biological Sciences,Agricultural and Biological Sciences (miscellaneous),Education

Reference11 articles.

1. Alozie, N.M., Grueber, D.J. & Dereski, M.O. (2012). Promoting 21st-century skills in the science classroom by adapting cookbook lab activities: the case of DNA extraction of wheat germ. American Biology Teacher, 74, 485–489.

2. Balgopal, M.M. (2010). Trail mix genetics: protein synthesis in two acts. Science Activities: Classroom Projects and Curriculum Ideas, 47, 22–28.

3. Banba, N., Katayama, T., Kozai, T. & Yonezawa, Y. (2013). A revision of the laboratory work on extraction of DNA at lower secondary school level [in Japanese with English abstract]. Japanese Journal of Biology Education, 53, 168–175.

4. Hearn, R.P. & Arblaster, K.E. (2010). DNA extraction techniques for use in education. Biochemistry and Molecular Biology Education, 38, 161–166.

5. Katayama, N., Takamori, H. & Kanaizuka, Y. (2004). Crisis of biology education in Japan. Asian Journal of Biology Education, 2, 75–80.

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