Population Growth in Euglena: A Student-Designed Investigation Combining Ecology, Cell Biology, & Quantitative Analysis

Author:

Oswald Christine1,Kwiatkowski Stephen2

Affiliation:

1. CHRISTINE OSWALD is a Professor at Southern Oregon University, 1250 Siskiyou Blvd., Ashland, OR 97520 oswald@sou.edu

2. STEPHEN KWIATKOWSKI is a Science Laboratory Preparator in the Biology Department at Southern Oregon University, 1250 Siskiyou Blvd., Ashland, OR 97520. e-mail: kwiatkowski@sou.edu

Abstract

We describe the use of Euglena gracilis for student-designed investigations of environmental factors that affect population growth of this unicellular flagellate. Each group of students can choose a topic of interest to them, but within the confines of a simple cell culture system that requires little space for each student group, and at a fairly low cost. Students are provided with stock cultures of E. gracilis, along with detailed instructions on preparing control medium and counting cells using a hemocytometer. Students then design their own experiments to test factors that possibly affect population growth over several weeks.

Publisher

University of California Press

Subject

General Agricultural and Biological Sciences,Agricultural and Biological Sciences (miscellaneous),Education

Reference9 articles.

1. Rapid ecotoxicological bioassay of nickel and cadmium using motility and photosynthetic parameters of Euglena gracilis;Environmental and Experimental Botany,2010

2. Using the green flagellate Euglena gracilis Klebs as physiological dosimeter: is a long-term bioassay more significant than a short-term one?;Turkish Journal of Botany,2001

3. Murashige T. , & SkoogF. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473—49.

4. Studies on Euglena and vitamin B12;Bulletin of the Torrey Botanical Club,1950

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