Studying Adenomatous Polyposis Coli (APC) gene at colorectal cancer patients. The roleof E2F transcription factor 1 (E2F1), tumor suppressor P14 (ARF) and marker ofproliferation Ki-67

Abstract

Purpose: The purpose of this study is to determinethe relationship between APC gene mutations andsubcellular differentiation levels of E2F1, P14ARFand KI67 proteins. Furthermore, if such connectionscan be used in the area of preventive health care.Materials and methods: The 30 hoursImmunohistochemistry protocol used, had samplespreparation, antigen retrieval, background blocking,target detection and sample visualization. Sampleswere viewed and captured by light microscopy.Results: The conducted research concern 88 patientswith a range age of 56 years. 57,7% had no tobaccoaddiction, 3,84% were obese and 19,23% had thetendency to consume alcohol. About 31% had atleast one family member with a history of cancer.Intensity and positivity of the genes vary as seen intables.Conclusions: By targeting specific andsimultaneously multiple pathways based onmolecular signatures, enables cases to be detected atan earlier stage, when there are greater chances ofcure as treatment is more effective. A plan for earlydetection of cancer is a key component within anoverall cancer control plan. An early diagnosisprogram is far more cheap and easy leading toappropriate treatments which finally reduce deathrates and suffering due to cancer