Clonal Analysis of Clinical and Environmental Pseudomonas aeruginosa Isolates from Meknes Region, Morocco

Author:

Maroui Itto1,Barguigua Abouddihaj2,Aboulkacem Asmae1,Elhafa Hanane1,Ouarrak Khadija3,Sbiti Mohammed4,Louzi Lhoussain4,Timinouni Mohammed2,Belhaj Abdelhaq1

Affiliation:

1. Ecology and Biodiversity of Wetlands Team, Department of Biology, Faculty of Sciences, Moulay Ismail University, Meknes, Morocco

2. Molecular Bacteriology Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco

3. Medical Biology Laboratory of Regional Hospital Mohammed V Meknes, Morocco

4. Medical Biology Laboratory of Regional Military Hospital Moulay Ismail Meknes, Morocco

Abstract

From 123 clinical and environmental Pseudomonas aeruginosa isolates, 24 strains were selected for their similar antibioresistance, virulence and biofilm formation profiles, to examine their diversity and occurrence of clones within two hospitals and different natural sites in Meknes (Morocco). Pulsed-field gel electrophoresis, using DraI enzyme, didn’t reveal a close relationship between clinical and environmental isolates nor between strains of the two hospitals. 19 genotypes were obtained, including two virulent environmental clones and three clinical clones virulent and resistant to antibiotics. Intra-hospital transmission of high-risk clones detected, in and between wards, constitutes a great public health concern.

Publisher

Polish Society of Microbiologists

Subject

Microbiology (medical),Applied Microbiology and Biotechnology,General Medicine,Microbiology

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