Assessment of DNA damage profile and oxidative/antioxidative biomarkers level in patients with inflammatory bowel disease

Author:

Mrowicka Małgorzata1,Mrowicki Jerzy1,Mik Michał2,Dziki Łukasz3,Dziki Adam2,Majsterek Ireneusz2

Affiliation:

1. Department of Clinical Chemistry and Biochemistry, Medical University, Lodz, Poland

2. Chair of Surgery, Department of General and Colorectal Surgery, Medical University, Lodz, Poland

3. Department of Clinical Nutrition and Gastroenterological Diagnostics, Medical 12 University, Lodz, Poland

Abstract

Aim: The purpose of this study was to investigate the oxidative DNA damage, pro- antioxidant status in Polish patients with inflammatory bowel disease (IBD). Method: Oxidative DNA damage were measured by comet assay techniques; nitric oxide (NO) and plasmatic lipid peroxidation (MDA) as oxidative stress were valuated by colometric methods; superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPx1) as antioxidative defence were determined by spectrophotometric methods. Results: The level of oxidative DNA damage in IBD patients was significantly higher in relation to controls (P = 0.01). Alike, in control subject as well as in patients with IBD, lymphocytes are characterized by complete repair of DNA damage. A significant decrease of SOD (P = 0.031), CAT (P = 0.006), GPx1 (P = 0.001) activity was seen in IBD patients vs control. MDA (P = 0.001) and NO (P = 0.001) concentrations were significantly increased in IBD patients than in healthy subjects. Conclusion: Our results may be due to induction of DNA repair genes may occur at the stage of the pathological changes pathway (IBD), that may be caused by excessive oxidative stress. However, the reasons for these relationship, and whether it is direct or indirect, remains to be explored.

Publisher

Index Copernicus

Subject

General Medicine,Surgery

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