Is leptin receptor expression triggered in case of embryo transfer to endometrium co-culture?

Abstract

Background/aim: A synchronized dialogue between maternal and embryonic tissues is required for a successful implantation. Low uterine receptivity is responsible for two-thirds of implantation failures and leptin is effective in physiology of reproduction by binding to specific receptors. In this study, we investigate leptin receptor expression in cases of embryo transfer to endometrial co - culture. Material and methods: Biopsy materials were taken from 20 females with indication for co - culture application and were cultured in appropriate medium, after the epithelial cells were isolated. The grown cells were cultured in chamber slides as the first group. For the second group, day 3 embryo was added to chamber slides and the development was observed. 1 - 2 days later, the embryo was transferred and other cells (after the transfer process) were used to form the second group. After fixation, immunohistochemical staining with anti-leptin primary antibody was done. Results: Regarding the co-culture without the embryo transfer, moderate leptin receptor immunoreactivity was seen in perinuclear region and in cell membrane. And regarding co-culture with the embryo transfer, moderate leptin receptor immunreactivity was seen in cytoplasm and strong leptin receptor immunoreactivity was seen in cell membrane. Conclusion: Embryo transfer to the endometrium co-culture triggers leptin receptor expression.