Author:
Korowash Sara Ibrahim,Abo-Almaged Hanan Hassan,Ibrahim Doreya M.
Abstract
Abstract
Hydroxyapatite (HA) is a biomaterial that has been widely studied for uses such as bone grafting and tissue engineering. Pure and hydroxyapatite nanoparticles with cationic (Na, Ti, and Li) as well as anionic (
CO
3
2
−
and Si4+) substitutions were synthesised using a wet chemical precipitation method followed by freeze-drying and investigated for their cytotoxicity effect. Inductively coupled plasma (ICP), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy were used to characterise the produced powders. The production of the HA phase was confirmed in all synthesised HA samples. MTT (3-(4.5-dimethylthiazol-2-yl)−2.5-diphenyl tetrazolium bromide) assay was performed using the BJ1 cell line of human normal fibroblasts to investigate the cytotoxicity of the synthesised HA samples. The cytotoxicity test showed that the HA samples were biocompatible, with the greatest results coming from Li-substituted (LiHA2) and Na-substituted (NaHA) hydroxyapatite.
Subject
Electrical and Electronic Engineering,Industrial and Manufacturing Engineering,General Materials Science
Reference40 articles.
1. Substituted hydroxyapatites for biomedical applications: a review;Šupová;Ceram. Int.,2015
2. Non-synthetic sources for the development of hydroxyapatite;Oladele;J. Appl. Biotechnol. Bioeng.,2018
3. Adhesion and osteogenic differentiation of human mesenchymal stem cells: supported by b-type carbonated hydroxyapatite;Sima,2012
4. Cationic and anionic substitutions in hydroxyapatite, Springer International Publishing Switzerland;Cacciotti,2016
5. Strontium doped hydroxyapatite from mercenaria clam shells: synthesis, mechanical and bioactivity study;Pal;J. Mech. Behav. Biomed. Mater.,2019
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献