Tailoring esophageal tumor spheroids on a chip with inverse opal scaffolds for drug screening

Author:

Shi RuolinORCID,Wu Xiangyi,Zhao Yuanjin,Gao Shegan,Liang Gaofeng

Abstract

Abstract Esophageal cancer (EC) is characterized by high morbidity and mortality, and chemotherapy has become an indispensable means for comprehensive treatment. However, due to the limitation of the effective in vitro disease model, the development of chemotherapeutic agents still faces great challenges. In this paper, we present a novel tumor spheroid on a chip platform based on inverse opal hydrogel scaffolds to screen chemotherapeutic agents for EC treatment. With the microfluidic emulsion approach, the inverse opal hydrogel scaffolds were generated with tunable and organized pores, which could provide spatial confinement for cell growth. Thus, the suspended KYSE-70 cells could successfully form uniform cell spheroids on the inverse opal hydrogel scaffolds. It was demonstrated that the tumor cell spheroids could recapitulate 3D growth patterns in vivo and exhibited higher sensitivity to the chemotherapy agents compared with monolayer cells. Besides, by employing the scaffolds into a microfluidics to construct esophageal tumor on a chip, the device could realize high-throughput tumor cell spheroids generation and drug screening, indicating its promising role in chemotherapy drug development.

Funder

National Key Research and Development Program of China

Key R&D project of Henan Province

Special Foundation for Basic Research Program of Higher Education Institutions of Henan Province

Innovation Scientists and Technicians Troop Construction Projects of Henan Province

Publisher

IOP Publishing

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