Abstract
Abstract
The cellular response of a normal human keratinocyte cell line exposed to non-cytotoxic doses of a deltamethrin-based pesticide was investigated by means of two different electrical impedance data spectroscopy approaches: Nyquist plot and broadband dielectric spectroscopy. The measurements have shown that the membrane capacity increases with pesticide concentration and this facilitates the electric current through cell membranes. Furthermore, the impedance of the extracellular matrix also increases with pesticide concentration, thus reducing the electric current outside the cell. Dielectric permittivity changes in the cellular samples at frequencies larger than 100 Hz. Fluorescence (FL) measurements emphasized an increase in neutral membrane lipids as a consequence of the pesticide exposure. Comparison of FL response of pesticide exposed cells with the control ones showed a time increase in the emission intensity, suggesting the existence of a membrane lipid response aimed at repairing the cell damage due to pesticide exposure. Therefore, both spectroscopic techniques have been demonstrated as potential means to investigate the response to cell stress and damage. This opens up new possibilities in the early diagnosis of cellular modifications related to the pesticide exposure of cells.
Funder
Università degli Studi di Bari
Instituto Nazionale di Fisica Nucleare Project ETHICS
Subject
Surfaces, Coatings and Films,Acoustics and Ultrasonics,Condensed Matter Physics,Electronic, Optical and Magnetic Materials
Reference44 articles.
1. Pesticides
2. Family pesticide use in the home, garden, orchard, and yard;Davis;Arch. Environ. Contam. Toxicol.,1992
3. Co-formulants in plant protection products. Initial study on the risk assessment of co-formulants in plant protection products. Project report on behalf of the FSVO;Schaller,2018
4. Cancer health effects of pesticides: systematic review;Bassil;Can. Fam. Phys.,2007
5. Developmental neurotoxicity of pyrethroid insecticides: critical review and future research needs;Shafer;Environ. Health Perspect.,2005
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献