Anticancer efficacy of lupeol incorporated electrospun Polycaprolactone/gelatin nanocomposite nanofibrous mats

Abstract

Abstract Despite the anticancer effect of lupeol (Lup), low aqueous solubility can make its therapeutic usage difficult. However, polycaprolactone/Gelatin (PCL-GEL) nanofibers scaffold eliminates this problem. This study has been conducted to recognize PCL-GEL-Lup nanofibers effect on cancer cell lines. PCL-GEL solution was prepared at different ratios (8 wt% and 4 wt%) for achieving optimal nanofibers. PCL-GEL-Lup nanofibers were provided via electrospinning technique. The surface morphology of nanofibers was examined using FESEM. Functional groups were investigated by a Fourier Transform Infrared spectroscopy. Lupeol released from nanofibers was detected by a UV–Visible spectroscopy. The drug release profile confirmed the sustained release of about 80% achieved within 40 h. IC50 of lupeol against ACHN and HSC-3 cell lines are 52.57 and 66.10 μg ml−1 respectively. The study results from aid an understanding of the fabrication of a scaffold with an optimum dose of bioactive lupeol in 6 wt% with bead free uniform diameter that is capable of binding the drug efficiently. The enhanced cytotoxicity activity by effective diffusion and elution to the target achieved in this study help to develop a nanofiber in the ongoing battle against cancer.