Multifunctional-high resolution imaging plate based on hydrophilic graphene for digital pathology

Author:

Lee Geonhee,Oh Yuna,Nam Jung Tae,Ji Seulgi,Jang A-Rang,Jeong Du Won,Kang MinSoung,Lee Sun Sook,Chae Soosang,Cho DonghwiORCID,Hwang Jun Yeon,Lee Kyungeun,Lee Jeong-OORCID

Abstract

Abstract In the present study, we showed that hydrophilic graphene can serve as an ideal imaging plate for biological specimens. Graphene being a single-atom-thick semi-metal with low secondary electron emission, array tomography analysis of serial sections of biological specimens on a graphene substrate showed excellent image quality with improved z-axis resolution, without including any conductive surface coatings. However, the hydrophobic nature of graphene makes the placement of biological specimens difficult; graphene functionalized with polydimethylsiloxane oligomer was fabricated using a simple soft lithography technique and then processed with oxygen plasma to provide hydrophilic graphene with minimal damage to graphene. High-quality scanning electron microscopy images of biological specimens free from charging effects or distortion were obtained, and the optical transparency of graphene enabled fluorescence imaging of the specimen; high-resolution correlated electron and light microscopy analysis of the specimen became possible with the hydrophilic graphene plate.

Funder

National Research Foundation of Korea

Focused Research Program of the Korea Research Institute of Chemical Technology

Publisher

IOP Publishing

Subject

Electrical and Electronic Engineering,Mechanical Engineering,Mechanics of Materials,General Materials Science,General Chemistry,Bioengineering

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