Silicon-assisted surface enhanced fluorescence toward improved assay performances

Abstract

Abstract A novel scheme of silicon-assisted surface enhanced fluorescence (SEF) is presented for SEF-based assays, where the blank signal suppression and the fluorescence signal enhancement is combined. The P-doped, (100) oriented silicon substrate is used to quench the fluorescence of Rose Bengal (RB) molecules attached to it, resulting in an effectively suppressed background signal, which is useful for a lower limit of detection (LOD). When a proper quantity of silver nanoparticles (AgNPs) is deposited on the RB-attached silicon substrate, a significant fluorescence enhancement of up to around 290 fold is obtained, which helps to improve the sensitivity in fluorescence-based assays. Besides, conventional gold nanoparticles (AuNPs) have also been demonstrated to exhibit excellent SEF effect using the presented scheme, providing improved stability and biocompatibility. The mechanism of the observed SEF effect has been investigated, and both the decreased apparent quantum yield and the silicon-induced electric field redistribution are considered to play important roles. The experimental results suggest that the presented scheme holds great potential in the SEF-based assays aiming at higher sensitivity and lower LOD.