Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA

Author:

Takahashi HiromiORCID,Yasui Takao,Kashida Hiromu,Makino Koki,Shinjo Keiko,Liu Quanli,Shimada Taisuke,Rahong Sakon,Kaji Noritada,Asanuma Hiroyuki,Baba Yoshinobu

Abstract

Abstract Detection of cell-free DNA (cfDNA) has an impact on DNA analysis in liquid biopsies. However, current strategies to detect cfDNA have limitations that should be overcome, such as having low sensitivity and requiring much time and a specialized instrument. Thus, non-invasive and rapid detection tools are needed for disease prevention and early-stage treatment. Here we developed a device having a microheater integrated with zinc oxide nanowires (microheater-ZnO-NWs) to detect target single-stranded DNAs (ssDNAs) based on DNA probe hybridization. We confirmed experimentally that our device realized in-situ annealed DNA probes by which we subsequently detected target ssDNAs. We envision that this device can be utilized for fundamental studies related to nanobiodevice-based DNA detection.

Funder

JSPS Grant-in-Aid for Scientific Research

Murata Science Foundation

Strategic International Research Cooperative Program

Japan Science and Technology Agency

JST PRESTO

the Nitto Foundation

the G-7 Scholarship Foundation

JSPS Grant-in-Aid for Exploratory Research

JSPS Grant-in-Aid for Scientific Research on Innovative Areas “Chemistry for Multimolecular Crowding Biosystems”

Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology

Cooperative Research Program of the

Advanced Technology Institute Research Grants 2019

Foundation of Public Interest of Tatematsu

Publisher

IOP Publishing

Subject

Electrical and Electronic Engineering,Mechanical Engineering,Mechanics of Materials,General Materials Science,General Chemistry,Bioengineering

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