DNA origami-based microtubule analogue

Abstract

Abstract A microtubule hollow structure is one type of cytoskeletons which directs a number of important cellular functions. When recapitulating biological events in a cell-free system, artificial frames are often required to execute similar cytoskeletal functions in synthetic systems. Here, I report a prototypical microtubular assembly using a DNA origami nanostructuring method. Through structural design at the molecular level, 32HB (helices bundle)-based DNA origami objects can form micrometers long tubular structures via shape-complementary side patterns engagement and head-to-tail blunt-end stacking. Multiple parameters have been investigated to gain optimized polymerization conditions. Conformational change with an open vs closed hinge is also included, rendering conformational changes for a dynamic assembly. When implementing further improved external regulation with DNA dynamics (DNA strand displacement reactions or using other switchable non-canonical DNA secondary structures) or chemical stimuli, the DNA origami-based microtubule analogue will have great potential to assemble and disassemble on purpose and conduct significantly complicated cytoskeletal tasks in vitro.