Novel Biodegradable Antimicrobial Composite Scaffold by Coating Type I Collagen Scaffold with Pepsin-Degraded SIS Extraction

Abstract

Abstract The antimicrobial activity of tissue engineering scaffolds is crucial for successful implantations and surgeries by reducing surgical infections. In this study, small intestinal submucosa (SIS) extracts were prepared by in vitro pepsin digestion (pSIS) and its antimicrobial active is stronger than those prepared by accelerated hydrolysis (aSIS). Compared with aSIS, the average molecular weight of pSIS seems smaller with more narrow distribution via SDS-PAGE electrophoresis combined with commassie blue staining. Furthermore, pSIS coated porous type I collagen scaffold (pSIS/COL I scaffolds) displayed a strong antibacterial activity and is suitable for cell seeded and growth, indicating that it can be potentially applied to tissue engineering or surgical tissue repairing in the future.