Inhibition Of Pseudomonas aeruginosa Virulence Factors Expression Regulated By Quorum Sensing System Using Ethyl Acetate Extract Of Temu Ireng (Curcuma aeruginosa)

Abstract

Abstract Pseudomonas aeruginosa is an opportunistic human pathogen that can cause serious problem in immunocompromised persons. Difficulty in treating P. aeruginosa infection is due to its abilities to develop multi drug resistence towards various antibiotic. Quorum sensing system is a potential target for P. aeruginosa infection because this system regulate the expression of virulence factors in this bacteria. In previous study, ethyl acetate extract of Temu Ireng rhizomes (Curcuma aeruginosa) was proved to inhibit quorum sensing in Chromobacterium violaceum. This study was aim to know the effect of C. aeruginosa extract towards expression of P. aeruginosa virulence factors which were regulated by quorum sensing, such as protease LasA activities, LasB and biofilm formation. Protease LasA activity assay was conducting using Staphylococcus aureus cell lysis activity. Protease LasB activity was measured by elastin congo red assay, and the formation of biofilm was monitored using PVC biofilm formation assay method. The result showed that ethyl acetate extract of C. aeruginosa rhizomes can decrease P. aeruginosa virulence controlled by quorum sensing, namely protease LasA, LasB, and biofilm formation by 66.92%, 37.8%, and 46% respectively.