Effect of Temperature and Length of Storage to Chloramphenicol Eye Drop’s Concentration

Abstract

Abstract Chloramphenicol is a broad spectrum antibiotic that acts by inhibiting protein synthesize. One of chloramphenicol dosage form which widely found in the marketplace is eyedrop. Drug storage will affect drug stability. This research aimed to know the effect of temperature and length of storage to chloramphenicol eye drop’s concentration. Analysis of chloramphenicol concentration in eyedrops was done by UV spectrophotometry. This analysis was done to eyedrops after stored 0; 7; 21; and 30 days unsealed with various storage condition: under the sunlight, room temperature, and refrigerated. Data obtained was analyzed statistically using one-way ANOVA with 95% CI by SPSS for windows software. The result showed that the concentration of chloramphenicol eyedrop before stored was to meet the requirements which were 95.96%. The result of chloramphenicol concentration in eyedrop after stored 30 days in 3 storage condition was reduced significantly. While sunlight and storage temperature did not affect the reduction of chloramphenicol concentration significantly.