Development of chitosan-hyaluronic acid nanoparticles and study of their physico-chemical properties for targeted delivery of anticancer drugs

Abstract

Abstract Nanoparticles from chitosan and hyaluronic acid were obtained using ionotropic gelation technology. The size of the nanoparticles was investigated using electron microscopy and dynamic light scattering. Nanoparticles were obtained of an optimal size of ∼ 100 nm. A physical association method has been developed of encapsulating nanoparticles with doxorubicin, a well-known antitumor drug, and dinitrosyl iron complex (donor of nitric oxide). The surface potential of nanoparticles was determined by dynamic light scattering. It was shown that HA-DOX: CS {6: 1} nanoparticles were stable and had a potential of -45.6 meV. The localization of nanoparticles in the cancer cells has been studied by confocal and FLIM microscopy. It was found that nanoparticles with doxorubicin are located in the cell near and inside the nucleus. It was shown that the encapsulation of DNIC in the composition of nanoparticles significantly increases the stability of DNIC, prolongs the formation and increases the yield of nitric oxide. A unique nano-system has been created for the delivery of anticancer drugs into the cell.