Evaluation of some Food Poisoning Bacterial Inhibition from ZnO and Ag Nanoparticles that Synthesized by Aspergillus niger

Abstract

Abstract The biosynthesis of ZnO and Ag nanoparticles (NPs) was conducted by using Aspergillus niger and determine the ability on the inhibition of E. coli and S. aureus. The morphology and particles sizes were found that ZnO and AgNPs at a rod to globular shapes, and the dimensions which were between 30 to 60 and 12 to 40 nm respectively. Also, determine the NPs synthesis absorbance of ZnO and AgNPs were to be at 368 and 420 nm respectively. The minimum inhibition concentration (MIC) assay was at 0.5, 1.0, 2.0, 5.0 10 and 20 % from each ZnO and AgNPs against S. aureus and E. coli have appeared that MIC act on inhibition for each bacterial growth at 1.2 %. and the Minimum Bactericidal Concentration (MBC) appeared at 20 mg/ml. The tested of 10, 15, 20 and 25 % from each ZnO and AgNPs on S. aureus and E. coli inhibition used wells method assay appear that inhibition diameter zone (IDZ) against S. aureus at 12, 13, 16 and 18 mm and 10, 14, 18 and 27mm respectively, While the IDZ against E. coli at 8, 10, 15 and 17 mm and 14, 16, 22 and 30 mm respectively. Thus, the ability of A.niger to synthesis of ZnO and AgNPs and these nanoparticles have the activity against bacterial species of S.aureus and E.coli.