Removal of crude oil by Thiobacillus sp. and Clostridium sp. at various temperatures and concentration of pollutant in liquid media

Abstract

Abstract The activities of the petroleum mining industry in Indonesia have caused many problems by producing hazardous waste pollution that would produce dangers for plants, animals or human lives. One technique to recover environmental situation is through land farming technique. The purpose of this study is to overcome crude oil pollution in the environment by using biotechnology method through the process of enzymatic bioremediation. The parameters discussed to determine the optimal environmental conditions that provide the highest efficiency for Total Petroleum Hydrocarbon (TPH) degradation processes such as temperature with a variation of 25 – 45°C then the concentration of pollutants with a variation of 5% – 20%, and the contact time carried out in solid media with variations amounting to 3 – 21 days. After conducting research, research that produces the bacterium Thiobacillus sp. and Clostridium sp. under optimal conditions at 35°C with a pollutant concentration of 10% and a contact time of 14 days. Thus, it can be concluded that the bacterium Thiobacillus sp. and Clostridium sp. can set aside hydrocarbon compounds with a removal efficiency of 65%. So, this research needs to be done to study other physical parameters.