Extraction and determination of alpha-Cypermethrin in environmental samples from Kerbala city / Iraq and in its formulation using high performance liquid chromatography (HPLC).

Abstract

Abstract Synthetic perothroid, Alpha-cypermethrin (α-CY), was determined in both river water samples collected from different agriculture areas in Kerbala city / Iraq and in some of its formulation which include α-cypermethrin as an active gradient. The method is based on the development of the analysis using high performance liquid chromatography (HPLC) with ultraviolet detection. Dispersive liquid-liquid microextraction (DLLME) was also developed to extract α-cypermethrin from river water samples by using chloroform as extraction solvent and acetonitrile (ACN) as dispersant. A mixture of acetonitrile/methanol (50:50v/v) was optimized as a mobile phase and C18 column (250mmx4.6mm,5μm) was chosen as a stationary phase, at pH 7.0 and flow rate 0.5 mL.min−1. Linearity of calibration curve ranged from (0.1-70)μg mL−1 was applied. Limit of detection and limit of quantification are (0.047) and (0.157)μg.mL−1, respectively. Validation of this method was maintained for three concentration (0.5, 10 and 50) μg mL−1. Relative standard deviation were (0.823, 0.113 and 0.150) μg.mL−1, respectively for the three level of concentrations. Three spiked level were applied for recovery study (1.0, 5.0, and 10) μg.mL−1. Recovery ranged from (83.704 - 96.972%) for the three spiked level. The developed method could be provide an acceptable results for determination of α-cypermethrin in its formulation and river water samples.