Anti-inflammatory Activity of Neuropathic Pain Reducing Herbal Medicine Based on Edema Inhibition of CARR-induced Sprague Dawley Paws

Abstract

Abstract Neuropathic pain is nerve pain whose treatment still has side effects when used for the long term. Regulation and modulation of the immune system is needed to reduce the inflammatory reaction that triggers of the pain. Increasing neural tension may induced with nerve inflammation which producing compounds responsible for signalling pain, fever and heat. Ginger (Zingiber officinale Roscoe), cloves (Syzygium aromaticum L.) and nutmeg (Myristica fragrans Houtt) have been known to contain phenolic compounds that are efficacious as anti-inflammatory agents. This study aims to prove the anti-inflammatory activity of neuropathic pain reducing herbs consisting of a combination of the three ingredients. Result from herbs formulation and reflux extraction then analysed for anti-inflammatory activity using in vivo & Winter method with Sprague-Dawley white male rats (Rattus norvegicus) and quantitative phenol content. Anti-inflammatory analysis done by 6 groups of rats, which is normal control, negative control, positive control, dosage I (given herbal extract 1.125 mL/200 g BW), dosage II (given herbal extract 2.25 mL/200 g BW) and dosage III (given herbal extract 4,5 mL/200 g BW). Anti-inflammatory of 10 g herbs extract in 250 mL solvent then observed through the inhibition of oedema formation on rats’ paw along the time. Dosage III group has the higher inhibition percentage as in 75.76%, which almost equivalent as normal control group (82.28%).