Preliminary Study of Gaultherin Extraction from Gandapura Through Uv-Photo Extraction

Author:

Sutrisno ,Ariwibowo D,Yulianto M E,Maulinda N A

Abstract

Abstract Gaultherin in gandapura can be extracted using hot water and calcium carbonate, followed by solvent extraction using acetic ester hydrate at a temperature of 100 °C. However, the process yields only 4 g/kg of fresh leaves. A technique of simultaneously gaultherase enzyme inactivation and gaultherin extraction with ethanol solvent should be studied. Preliminary studies of Gaultherin extraction with ethanol has been done and it is able to obtain gaultherin up to 14.46% at pH 8. This study will examine the development of the process photo-extraction-UV and osmosis dehydration simultaneously. The advantages of this process are (i) the gaultherase enzyme experiences unfolding, resulting in reduced hydrolysis reaction, (ii) increase in gaultherin yield, (iii) needs less energy, and (iv) purification of the product would be lighter. Study of the effect of solvent concentration and extraction time on gaultherin recovery was carried out for 2 months in the Laboratory of Chemical Engineering Operations at UNDIP Vocational School. Variables include dependent and independent variables. Dependent variables include the solvent-feed ratio of 5:1, pH 4.8, 75 rpm of mixer speed, chopper blades speed of 125 rpm, a drying temperature of 80 °C. Independent Variables include ethanol concentration at 80, 85, 90, and 95%. Extraction time for 10, 20, 30, 40 and 50 minutes. The result shows that gandapura extraction using ethanol had dual function, inactivate gaultherase enzymes and extract the active compounds gaultherin. Gaultherin yields was increased by increasing the duration of extraction. Whereas increasing the concentration of the solvent, gaultherin extracted was decreased.

Publisher

IOP Publishing

Subject

General Medicine

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1. Response surface methodology on the optimization gaultherin production from gandapura (Gaultheria fragrantissima) UV-photo extraction;THE 6TH INTERNATIONAL CONFERENCE ON ENERGY, ENVIRONMENT, EPIDEMIOLOGY AND INFORMATION SYSTEM (ICENIS) 2021: Topic of Energy, Environment, Epidemiology, and Information System;2023

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